FIGURE 6.

TNF-α induction of S100A6 in cardiac myocytes. A, representative Western blots of S100A6 andβ-actin in myocyte cultures transfected with either vector alone or the human S100A6 expression plasmid or an siRNA duplex against S100A6 and treated for 48 h with either vehicle or TNF-α (5 ng/ml). Respective densitometry units relative to vehicle control are displayed below blots. ^*, p < 0.05 versus vehicle and vector.!, p < 0.05 versus TNF-α and vector. n = 3. B, myocyte cultures were transfected with either a human S100A6 expression plasmid or an siRNA duplex against S100A6 or empty vector and treated for 48 h with either PDGF (5 ng/ml), TNF-α (5 ng/ml), or vehicle. Myocytes were assessed for caspase-3 activity by the EnzChek caspase-3 assay kit. The results are the mean ± S.E. of 6–8 different experiments. ^*, p < 0.05 versus vehicle and/or vector.!, p < 0.05 versus TNF-α and vector alone.