TABLE 1.
DTT dose-dependent inactivation of caspase-3 by 0.1 μm compound III The half-life (t½) for the inactivation at given condition was defined as t½ = 0.693/kobs.
|
DTT
|
t½
|
||
|---|---|---|---|
| 2% DMSO | 0.1 μm Compound III | ||
| mm | min | ||
| 0.00 | 61.8 ± 1.0 | 62.3 ± 3.6 | |
| 0.02 | 67.1 ± 4.4 | 60.8 ± 0.2 | |
| 0.04 | 90.8 ± 0.8 | 49.7 ± 3.8 | |
| 0.08 | 546.9 ± 43.7 | 41.2 ± 4.6 | |
| 0.16 | 719.6 ± 14.1 | 26.6 ± 1.8 | |
| 0.31 | 825.7 ± 19.0 | 12.0 ± 2.5 | |
| 0.63 | 933.6 ± 83.9 | 8.9 ± 0.6 | |
| 1.25 | 1352.8 ± 54.4 | 4.7 ± 0.2 | |
| 2.50 | 1428.9 ± 122.1 | 4.5 ± 0.1 | |
| 5.00 | 1799.0 ± 118.3 | 4.3 ± 0.3 | |
| 10.00 | 1940.6 ± 46.3 | 3.3 ± 0.5 | |
| 20.00 | 2528.1 ± 18.9 | 2.4 ± 0.0 | |