FIGURE 10.

15d-PGJ₂ induces a JNK-dependent p53 phosphorylation at Ser-392 in vascular ECs. Animal treatment is described under “Experimental Procedures.” A, after treatment for 8 h, corneas were harvested and subjected to immunofluorescent assay for eNOS (red) and p-p53 (Ser-392) (green). Nuclei were stained by Hoechst 33342 (blue). Magnification, ×40. Merged images indicate that 15d-PGJ₂ could markedly induce p-p53 (Ser-392) and that the effect was attenuated by the JNK inhibitor but not by the p38 MAPK inhibitor. B, percentages of eNOS-positive EC with p-p53-positive nuclei. ^*, p < 0.05 versus PBS-treated corneas. #, p < 0.05 versus 15d-PGJ₂-treated corneas.