FIGURE 6.

15d-PGJ₂ dose- and time-dependently induces JNK and p38 MAPK phosphorylation. A, HUVECs were exposed to 15d-PGJ₂ for the indicated time periods. Western blotting was performed to detect the active phosphorylated forms of JNK (p-JNK) and p38 MAPK (p-p38) and is shown in the upper panels. Antibodies were then stripped and re-incubated with anti-JNK and anti-p38 MAPK antibodies (lower panels), respectively, to detect the levels of total JNK and p38 MAPK. B, HUVECs were exposed to 1-20 μm of 15d-PGJ₂ for the times indicated. Western blotting was performed as described above. C, intensities of p-JNK1/2, p-p38 MAPK, and p53 were determined by densitometry.