FIGURE 7.

15d-PGJ₂ induces JNK and p38 MAPK phosphorylation in vascular ECs. Mouse corneas were subjected to alkali trauma and treated 3 days later with either 20 μl of PBS or 20 μm 15d-PGJ₂. After treatment for 8 h, corneas were harvested and subjected to immunofluorescent assay for eNOS (red; specific for vascular endothelium) and p-JNK or p-p38 MAPK (green). Nuclei were stained by Hoechst 33342 (blue). p-JNK and p-p38 MAPK localized in the nuclei (pale blue) and co-localized with eNOS (yellow) are shown in merged images. Magnification ×40. Bar, 75 μm.