FIGURE 1.

Identification of conserved residues in the S1-S2 linker of the Kv family. Upper panel, schematic diagram of the predicted membrane topology for a Kv1 family subunit, including the N-linked glycosylation (NLG) site found in the Kv1.4 S1-S2 linker and the T1 domain required for tetramerization. The asterisk denotes conserved threonine (residue 330 in Kv1.4). Lower panel, ClustalW sequence alignment of the S1-S2 extracellular linker of the rat Kv1 family and further alignment of other Kv families (beginning from the proposed last residue (leucine (L) or equivalent, numbered at left) emerging from the S1 transmembrane domain. Conserved residues are shown by asterisks with the conserved threonine boxed. Numbers in parentheses indicate the lengths of each S1-S2 linker. All sequences are from rat except Kv4.1, which is from mouse. (Evolutionary conservation of the S1-S2 linker region is shown in Fig. 9).