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Mutations of threonine to either a serine (eYFP-Kv1.4:T330S) or an aspartic acid (eYFP-Kv1.4:T330D) indicate a requirement for a hydroxyl group. HEK293 cells (A and B) and COS-7 cells (C and D) transiently expressing eYFP-Kv1.4:T330S (A and C) or eYFP-Kv1.4:T330D (B and D) were fixed at 48 h post-transfection and viewed under a ×100 objective using a DeltaVision work station. Green, eYFP fluorescence; blue, DAPI (nuclei). Scale bar, 15 μm.
