Figure 2.

Stimulation of GAS reporter activity by ICSBP. (a) RAW or CL-2 cells were cotransfected with the WT GAS or mt GAS reporter as described for Fig. 1d, along with the ICSBP vector or empty vector, and treated with or without IFNγ as described for Fig. 1. (b) Absence of ISRE-reporter stimulation by ICSBP. Cells were cotransfected and assayed as described for a, except that the ISRE luciferase reporter was used. (c) RAW cells were cotransfected with WT GAS reporter and empty vector, vector for IRF-1, IRF-2, or ICSBP, and then luciferase activity was measured as described for a. (d) ICSBP domain analysis. RAW cells were cotransfected with the WT GAS reporter and indicated deletion constructs and assayed as described for a. DBD, DNA-binding domain; CTD, C-terminal domain; FL, full length.