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. 2008 Nov 7;283(45):30438–30450. doi: 10.1074/jbc.M802219200

FIGURE 1.

FIGURE 1.

Identification of the transcriptional start sites of the rpoS gene in V. vulnificus. A, primer extension experiment using V. vulnificus RNA and the oligonucleotide primer PE+83 (annealing to the region from +54 to +83 relative to the IC of rpoS). Lanes C, T, A, and G represent the nucleotide sequencing ladders of pINE32. The asterisk indicates the site of transcriptional initiation for rpoS. B, primer extension experiment using the oligonucleotide primer PE-368 (annealing to the region from –368 to –339 relative to IC) as described above. C, two rpoS promoters in the nlpD open reading frame and nlpD-rpoS intergenic space. The promoter Pp is proximal to the IC of rpoS, and the promoter Pd is distal from the IC of rpoS. The promoters, –10 and –35 regions, are underlined, whereas the stop codon of nlpD and the start codon of rpoS are capitalized.