TABLE 3.
Expression of rpoS::luxAB fusions in various genetic backgrounds
|
Genetic background
|
Intracellular cAMP
levela
|
Expression of luxAB-transcriptional fusion
(RLU/OD)b
|
|
|---|---|---|---|
| pKP-368 | pKP-1315 | ||
| fmol/μg protein | |||
| Wild type | 8.4 | 1,320 ± 86 | 267,780 ± 1,450 |
| crp | 421 | 3,430 ± 95 | 739,960 ± 87,700 |
| Δcya | Not detectablec | 3,130 ± 26 | 632,590 ± 17,900 |
| Δcya + cAMP (0.5 mm) | ndd | 1,110 ± 9 | 219,540 ± 9,080 |
| ΔcpdA | 24 | 590 ± 16 | 119,480 ± 4,610 |
a
cAMP contents were measured from the stationary phase V. vulnificus, and its levels were expressed as moles of cAMP in the unit mass of bacterial cells (determined by protein amount in lysate).
b
RLU per cell mass determined by A595.
c
cAMP concentration was below the detection limit of the assay used in this study.
d
ND means not determined.