FIGURE 2.

Relationship between structure and activity of primate LL-37 orthologs. For each peptide, the percentage of helix in DMEM (A) and the percentage of lactate dehydrogenase (LDH) release (B) was plotted versus the corresponding increase in proliferation of NIH 3T3 cells. The ellipticity in DMEM was taken from Table 1. Lactate dehydrogenase release was calculated as the percentage of the lactate dehydrogenase activity in the cell supernatant over total cellular activity after 90-min incubation with each peptide (5 μm). The percent increase in cell proliferation after treatment with each peptide (5 μm) was calculated by the MTT assay as described above. Data are the mean of at least three separate experiments. Linear regression was applied to the data to allow calculation of correlation coefficients, R².