FIGURE 6.
LL-37 potentiates the [Ca2+]i rise mediated by BzATP in HEK293-hP2X7 cells. A, fura-2/AM-loaded HEK293-hP2X7 cells were incubated for 6 min with 1 μm LL-37 (trace a) or 1 μm mmu RL-37 (trace b) and then stimulated with 30 μm BzATP. As a control, BzATP was added in the absence of preincubation with either peptide (trace c). Cells pretreated with LL-37 were stimulated with BzATP also in the presence of 100 nm KN-62 (trace d). The black arrow indicates the point of BzATP addition. B, effect of increasing concentrations of LL-37 on 30 μm BzATP-mediated [Ca2+]i increase. The [Ca2+]i peak (closed squares) and the following plateau (open squares) were measured 2 and 8 min after BzATP addition, respectively. Data are averages ± S.D. of three independent experiments.