FIGURE 2.

KIV₁₀ is required for apo(a)-induced cytoskeletal reorganization in HUVECs. HUVECs were serum-starved for 15 min (A) and then treated with 200 nm of r-apo(a) variants (17K, IV_1–4, KIV₅-P, KIV_5–10, and KIV₁₀-P) for 20 min (B–F, respectively). Cells were fixed, permeabilized, and stained for F-actin (left) and VE-cadherin (right) using TRITC-phalloidin and a monoclonal anti-VE-cadherin antibody followed by an Alexa-488-linked secondary antibody, respectively.