FIGURE 2.

Production, expression, and analysis of TRAP-Ostm1 transgenic mice. A, schematic representation of the transgene used to generate TRAP-Ostm1 mice. The TRAP regulatory sequences (1.3 kb), transcription initiation site (+1), first exon (500 bp), and intron 1 were cloned upstream of Ostm1 (1.055 kb) open reading frame followed by the 3′ and poly(A) of human growth hormone gene (hGH; 2.1kb). B, transgene expression in OCLs (TR) from each TRAP-Ostm1 transgenic lines was compared with non-transgenic OCL controls (NT). Endogenous Trap, Ostm1, and β-actin expression were used as controls. C, persistence of osteopetrosis was observed in homozygous gl/gl TRAP-Ostm1 transgenic mice (gl/gl TR) similar to gl/gl non-transgenic littermate (gl/gl NT) in contrast to wild-type control (+/+) as detected by x-ray analysis (top panels) and on histological bone sections (bottom panels, magnification, ×50).