TABLE 1.
Summary and relative amounts of detected PrPSc truncated fragments in the CJD subtypes
Quantification was performed by densitometric analyses of chemiluminescence Western blot signals generated by PNGase F-treated samples probed either with SAF60 or 2301 antibodies for detection of PrP27–30AF, CTF 13 or with CTF 12/13, DCF 16, respectively. The amounts are expressed as a percentage of PrP27–30. Each value represents the mean ± S.E. The number of cases analyzed for each group is reported under “Experimental Procedures.”
PrP27-30 | PrP27-30AF | DCF 16a | CTF 13 | CTF 12/13 | |
---|---|---|---|---|---|
MM/MV1 | 100 | 28.0 ± 3.9 | 31.3 ± 6.0 | 20.3 ± 3.3 | 22.3 ± 2.5 |
VV1 | 100 | <1b | NDc | 20.8 ± 8.7 | 72.6 ± 13.6 |
VV2 | 100 | 12.2 ± 4.0 | ND | ND | 19.2 ± 9.0 |
MV2 | 100 | 12.2 ± 6.4 | ND | ND | 21.7 ± 16.6 |
MM2-C | 100 | 17.4 ± 3.5 | ND | ND | 2.6 ± 1.7 |
MM2-T | 100 | 1.3 ± 1.0 | ND | 4.2 ± 1.3 | 16.9 ± 7.3 |
vCJD | 100 | 36.2 ± 6.2 | ND | ND | ND |
DCF 16 = the 16-kDa PrPSc fragment generated by PK digestion performed in partially denaturing conditions.
Only traces were detectable.
ND, not detectable.