TABLE 1.

Summary and relative amounts of detected PrP^Sc truncated fragments in the CJD subtypes

Quantification was performed by densitometric analyses of chemiluminescence Western blot signals generated by PNGase F-treated samples probed either with SAF60 or 2301 antibodies for detection of PrP27–30_AF, CTF 13 or with CTF 12/13, DCF 16, respectively. The amounts are expressed as a percentage of PrP27–30. Each value represents the mean ± S.E. The number of cases analyzed for each group is reported under “Experimental Procedures.”

	PrP27-30	PrP27-30AF	DCF 16a	CTF 13	CTF 12/13
MM/MV1	100	28.0 ± 3.9	31.3 ± 6.0	20.3 ± 3.3	22.3 ± 2.5
VV1	100	<1b	NDc	20.8 ± 8.7	72.6 ± 13.6
VV2	100	12.2 ± 4.0	ND	ND	19.2 ± 9.0
MV2	100	12.2 ± 6.4	ND	ND	21.7 ± 16.6
MM2-C	100	17.4 ± 3.5	ND	ND	2.6 ± 1.7
MM2-T	100	1.3 ± 1.0	ND	4.2 ± 1.3	16.9 ± 7.3
vCJD	100	36.2 ± 6.2	ND	ND	ND

^aDCF 16 = the 16-kDa PrP^Sc fragment generated by PK digestion performed in partially denaturing conditions.

^bOnly traces were detectable.

^cND, not detectable.