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. 2008 Nov 7;283(45):30557–30565. doi: 10.1074/jbc.M801877200

TABLE 1.

Summary and relative amounts of detected PrPSc truncated fragments in the CJD subtypes

Quantification was performed by densitometric analyses of chemiluminescence Western blot signals generated by PNGase F-treated samples probed either with SAF60 or 2301 antibodies for detection of PrP27–30AF, CTF 13 or with CTF 12/13, DCF 16, respectively. The amounts are expressed as a percentage of PrP27–30. Each value represents the mean ± S.E. The number of cases analyzed for each group is reported under “Experimental Procedures.”

PrP27-30 PrP27-30AF DCF 16a CTF 13 CTF 12/13
MM/MV1 100 28.0 ± 3.9 31.3 ± 6.0 20.3 ± 3.3 22.3 ± 2.5
VV1 100 <1b NDc 20.8 ± 8.7 72.6 ± 13.6
VV2 100 12.2 ± 4.0 ND ND 19.2 ± 9.0
MV2 100 12.2 ± 6.4 ND ND 21.7 ± 16.6
MM2-C 100 17.4 ± 3.5 ND ND 2.6 ± 1.7
MM2-T 100 1.3 ± 1.0 ND 4.2 ± 1.3 16.9 ± 7.3
vCJD 100 36.2 ± 6.2 ND ND ND
a

DCF 16 = the 16-kDa PrPSc fragment generated by PK digestion performed in partially denaturing conditions.

b

Only traces were detectable.

c

ND, not detectable.