Effects of MCT-1 on undifferentiated or differentiated HL-60 cells.
A, β-hexosaminidase release upon stimulation with various
concentrations of fMLF (left) or MCT-1 (right) from the
neutrophilic/granulocytic differentiated HL-60 cells following treatment with
500 μm dbcAMP for 24 (open triangles), 48 (closed
triangles), or 72 h (open squares). The effects of the peptides
on the undifferentiated cells were also measured (filled circles).
B, [Ca2+]i increase in
undifferentiated (filled bars) and differentiated (treated with 500
μm dbcAMP for 72 h; open bars) HL-60 cells after
stimulation with vehicle, fMLF, or MCT-1. The effect of ATP was also
determined as the control known to be unaffected by differentiation. All data
are expressed as mean ± S.E. of six independent experiments.