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Kinetic parameters for rDer p 3 in the presence of 50 mm polybuffer, pH 8.5
| Substrate | Km | kcat | kcat/Km |
|---|---|---|---|
| μm | s–1 | 105m–1 s–1 | |
| Boc-IEGR-AMC | 27 ± 1 | 17.3 ± 0.2 | 6.4 ± 0.3 |
| Boc-GPR-AMC | 14 ± 1 | 12.4 ± 0.2 | 8.9 ± 0.7 |
| Boc-FSR-AMC | 11 ± 1 | 9.9 ± 0.3 | 9 ± 0.9 |
| Boc-QAR-AMC | 33 ± 3 | 8.6 ± 0.2 | 2.6 ± 0.2 |
| NAR-ACC | 190 ± 30 | 70 ± 3 | 3.7 ± 0.6 |
| NAT-ACC | NCa | NC | NC |
NC means not cleaved
