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. 2008 Nov 7;283(45):30632–30641. doi: 10.1074/jbc.M801401200

FIGURE 5.

FIGURE 5.

GAPDH reactivates the AP endonuclease activity of oxidized APE1. A fixed amount (2.7 pmol) of oxidized His-APE1oxd was incubated with increasing concentrations of either purified GST (lanes 10–12) or purified GST-GAPDH (lanes 13–16) for 5 min at room temperature and then assayed for AP endonuclease activity. Shown are the controls, substrate (80 ng) alone (lane 1) and the following purified proteins Endo IV (0.3 pmol, lane 2), His-APE1 (lanes 3–5), oxidized His-APE1oxd (lanes 6 and 7), GST (lane 8), and GST-GAPDH (lane 9).