FIGURE 6.

GAPDH depletion influences survival of mammalian cells following exposure to genotoxic agents. A, cells were incubated with 75 nm of either control siRNA or GAPDH siRNA and total cell extracts analyzed for GAPDH and APE1 levels by Western blot analysis. B–D, control and siRNA-treated cells were challenged with increasing doses of either MMS (B), bleomycin (C), or UVC (D) and monitored for survival by clonogenic assay. The means and standard deviations of at least three independent experiments are shown.