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Aminoacylation assays for Wt and mutant yeast ValRSs. A, aminoacylation assay. The aminoacylation activity of the Wt and mutant ValRSs was tested at a final concentration of 5 nm. The relative amounts of [3H]valine that were incorporated into tRNA were subsequently determined by a liquid scintillation counter. B, aminoacylation assay. The aminoacylation activity of the mutant ValRSs was tested at a final concentration of 200 nm.
