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. 2008 Nov 7;283(45):30699–30706. doi: 10.1074/jbc.M805339200

FIGURE 4.

FIGURE 4.

Gel mobility-shift assays for the tRNA binding activities of the Ad and Wt and mutant yeast ValRSs. Affinities of the Ad and Wt and mutant ValRSs toward 32P-labeled unfractionated yeast tRNA or in vitro-transcribed yeast tRNAVal were determined by polyacrylamide gel coelectrophoresis. At the bottom of each gel are the concentrations of the protein used. As a control, the left-most lane in each gel contained no protein. A, Ad binding unfractionated yeast tRNA. B, Ad binding yeast tRNAVal. C, Wt binding unfractionated yeast tRNA; D, Wt binding yeast tRNAVal. E, Δ1–97 binding yeast tRNAVal; F, Δ32–71 binding yeast tRNAVal.