FIGURE 5.
Converting E. coli GlnRS into a functional yeast enzyme. Arc1p and the Ads of yeast GlnRS, yeast ValRS, and human ValRS were independently fused at the N terminus of E. coli GlnRS, and the ability of the fusion enzymes to rescue the growth defect of a yeast GLN4 knock-out strain was tested. A, complementation assays for cytoplasmic GlnRS activity on a 5-FOA plate. B, E. coli GlnRS binding unfractionated yeast tRNA. C, E. coli GlnRS binding yeast tRNAGln. D, Ad(ScValRS)-EcGlnRS binding unfractionated yeast tRNA. E, Ad(ScValRS)-EcGlnRS binding yeast tRNAGln. F, Ad(ScValRS)-EcGlnRS binding yeast tRNAPhe.