Height and amplitude AFM topographs of flattened membrane patches.
A, a height image of three membrane pieces. Section 1 shows
that the upper left patch is essentially flat and well adsorbed to the mica
with the contours of LH2 complexes defined in the trace, whereas section
2 across the lower patch has significantly more vertical topology yet
still demonstrates the zigzag lines of complexes, which are clear in the
amplitude image (B). A region of empty lipid is denoted by the
black asterisk. Full gray scale, 20 nm. B, the amplitude
image of the same region showing that even the highly curved membrane patch
still has the regular zigzag lines of LH2 complexes traversing it.