TABLE 2.
Summary of published kinetic parameters for FAD synthetases The top of the table shows kinetic parameters for prokaryotic FAD synthetases, which share sequence similarity to AtRibF1 and AtRibF2. The bottom of the table shows kinetic parameters for mammalian FAD synthetases, which share no sequence similarity to AtRibF1, AtRibF2, or prokaryotic FAD synthetases.
| Organism (substrate) | Km | Ki × 103 | kcat × 10–2 | Ref. |
|---|---|---|---|---|
| μm | μm | s–1 | ||
| C. ammoniagenes (FMN) | 1 | 30 | Efimov et al. (1) | |
| C. ammoniagenes (ATP) | 37 | 0.0216 | 30 | Efimov et al. (1) |
| 160 | 45 | Manstein and Pai (3) | ||
| B. subtilis (FMN) | 0.38 | Kearney et al. (5) | ||
| B. subtilis (ATP) | 300 | Kearney et al. (5) | ||
| Rat (FMN) | 9.1 | Yamada et al. (15) | ||
| 9.6 | Oka and McCormick (17) | |||
| Rat (ATP) | 71 | Yamada et al. (15) | ||
| 53 | Oka and McCormick (17) | |||
| Human 1 (FMN) | 1.5 | Brizio et al. (19) | ||
| Human 2 (FMN) | 0.4 | 0.36 | Galluccio et al. (20) |