FIGURE 1.

Characterization of the promoter activity of the PED/PEA-15 5®-flanking region. The PED/PEA-15 5′-flanking fragments were cloned upstream to a promoterless luciferase reporter gene in the pGL3 Basic vector as described under “Experimental Procedures”. HeLa (black bars) and HEK 293 cells (gray bars) were then cotransfected with 3 μg of the construct DNAs (or 3 μg of the promoterless pGL3 Basic vector DNA) and 1 μg of the pRSVβ-gal vector DNA. Luciferase activity was assayed as described under “Experimental Procedures” and is presented as the increase above the activity measured with the pGL3 Basic vector. The results are presented as the means (normalized for β-galactosidase activity) ± S.D. of four independent experiments each performed in quadruplicate. Asterisks denote statistically significant differences (p < 0.001).