FIGURE 7.

Phenotypic characterization of ALase-silenced Euglena cells. A, growth phenotype of dsRNA-introduced cells in a medium supplemented with l-GalL. Cells were restored and grown for 1 week in the presence of 1 mm l-GalL after the introduction of dsRNAs for ALase. Cultures of representative cells grown in a medium supplemented with 1 mm l-GalL were photographed 1 week after the dsRNA was introduced. B, Northern blot analysis. Each lane was loaded with 10 μg of total RNA from 1-week-old wild type (WT) cells and dsRNA-introduced cells. The RNA blot was hybridized with a ³²P-labeled ALase cDNA probe. The bottom shows ethidium bromide-stained rRNA as a RNA-loading control.