FIGURE 9.

AsA level and expression of ALase under light in dark-grown Euglena. The cells were illuminated at an intensity of 55 μmol m^-2 s^-1. Samples were taken out at the indicated times and used for the determination of AsA, the assay of ALase, and Northern hybridization. Values are expressed as the mean ± S.E. for three experiments. A, AsA level. B, ALase activity. C, Northern blot analysis. Ten micrograms of total RNA extracted from each sample was electrophoresed through a formaldehyde-containing agarose gel, capillary blotted onto a nylon membrane, and hybridized with ³²P-labeled cDNA of Euglena ALase. Ethidium bromide staining of the rRNA is shown for the equality of loading. Values that were significantly different between control and illuminated cells are indicated. ^a, p < 0.05; ^b, p < 0.01.