TABLE 1.

Comparison of substrate specificities of the recombinant Euglena ALase with the homologous enzyme from rat

All substrates were at a concentration of 5 mm. Data are the means of three independent determinations ± S.E.

Substrate	Specific activity
	rEgALase	SMP30a
	μmol min-1 mg-1 of protein
d-Glucono-δ-lactone	39.0 ± 0.8 (100%)	217 ± 3.6 (100%)
l-Glucono-δ-lactone		5.4 ± 2.2 (2.49%)
l-Galactono-γ-lactone	24.5 ± 3.3 (62.8%)	0.8 ± 0.0 (0.37%)
d-Galactono-γ-lactone	27.3 ± 2.3 (70.0%)	9.4 ± 0.3 (4.33%)
l-Gulono-γ-lactone	40.9 ± 0.1 (105%)	2.7 ± 0.1 (1.24%)
d-Gulono-γ-lactone	20.1 ± 0.5 (51.5%)	12.0 ± 0.9 (5.53%)

^aKondo et al. (6).