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Effect of various compounds on purified recombinant ALase from Euglena
The values are the mean ± S.E. for three independent experiments.
|
Compound |
Concentration |
Relative activity |
|
|---|---|---|---|
| l-GalA | l-GalL | ||
| mm | % | ||
| None | 100 | 100 | |
| EDTA | 1 | 1.2 | 0.0 |
| AsA | 1 | 94.7 | 98.8 |
| Dithiothreitol | 1 | 97.3 | 105 |
| H2O2 | 0.1 | 123 | 118 |
| 0.5 | 132 | 127 | |
| N-Ethylmaleimide | 0.4 | 52.5 | 0.0 |
| 4.0 | 33.3 | 0.0 | |
