FIGURE 10.

LjIDP1 prevents dehydration- and freezing-induced enzyme inactivation. A, residual activity of CS (250 μg/ml) before and after repeated cycles of dehydration/rehydration either alone of in the presence of stabilizing agents. C, similar experiment with LDH (100 μg/ml). LjIDP1 effectively prevents inactivation of CS and LDH even after four cycles of dehydration/rehydration. B, residual activity of CS (250 μg/ml) before and after repeated cycles of freezing/thawing, either alone or in the presence of stabilizing agents. D, similar experiment with LDH 100 μg/ml. CS alone is only slightly affected by freezing, and after eight cycles of freezing/thawing, it retains ∼60% activity. In contrast, LDH alone quickly loses activity upon freezing, but adding LjIDP1 effectively prevents this. Numbers on the x axis refer to the number of cycles. The activity at the start (cycle 0) of the experiments is set to 100%. Error bars, 95% confidence intervals.