FIGURE 5.

Mixtures of Mcm4, Mcm6, and Mcm7 catalyze the hydrolysis of ATP. A, Mcm proteins were incubated with [γ-³²P]ATP at 37 °C for the time points indicated, and the reaction products were then analyzed by thin layer chromatography. The experiments were conducted in the absence (-DNA) and presence (+DNA) of 1 μm 60-dT ssDNA. B, data from experiments in A were quantified, and the rates of ATP hydrolysis were determined by a linear fit of the data. Data for time 0 were subtracted as background, and the data shown are the calculated ATP hydrolysis rates + error. The inset allows the comparison of Mcm mixtures with slower ATP hydrolysis rates. Striped bars indicate the absence of DNA, and open bars indicate the presence of 60(dT) ssDNA.