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. 2008 Nov 7;283(45):31183–31196. doi: 10.1074/jbc.M801606200

FIGURE 1.

FIGURE 1.

Effects of steroid hormones on cell migration and the actin cytoskeleton in A549 cells. A, effects of steroid hormones on cell migration. A549 cells were cultured with the indicated steroid hormones for 48 h and treated with mitomycin C for 2 h preceding the assay. Migration toward FBS in the presence of the steroids was measured in a Transwell migration chamber (mean ± S.D., **, p < 0.01 compared with vehicle; n.s., not significant). B, effect of steroid hormones on the actin cytoskeleton. A549 cells were treated with 1 μm of each steroid for 48 h and stained with phalloidin (red in merged image) and anti-vinculin antibody (green in merged image). Bar, 50 μm. C, effect of DEX on Rho activity. The Rho activity was determined by RBD-beads pulldown assay as described under “Experimental Procedures.” The amounts of total and GTP-RhoA protein were detected by Western blot with anti-RhoA antibody. The amounts of Rho downstream effectors, ROCK1, ROCK2, mDia1, cofilin 1, phospho(Ser-3)-cofilin 1, MLC2, phospho(Ser-19)-MLC2, and profilin were also analyzed by Western blot analysis using their corresponding antibodies.