Skip to main content
. 2008 Nov 7;283(45):31183–31196. doi: 10.1074/jbc.M801606200

FIGURE 4.

FIGURE 4.

GR up-regulates the expression of CaD in A549 cells. A, effect of GR or SRF depletion on the DEX-induced up-regulation of CaD expression. A549 cells transfected with control siRNA, GR siRNA, or SRF siRNA were incubated with vehicle or 1 μm DEX for 48 h. The expression levels of GR, SRF, and CaD were measured by Western blot analysis. B, CaD mRNA expression in the GR- or SRF-depleted cells was measured by real time qPCR (mean ± S.D., **, p < 0.01). C, effect of the expression of GRΔC on the CaD mRNA level in A549 cells transiently transfected with pCS2+GRΔC-cFLAG or the empty vector. The cells were incubated for 24 h. The CaD mRNA levels were measured by real time qPCR (mean ± S.D., ***, p < 0.001). D, expression levels of CaD and GRΔC in GFP- or GRΔC-cFLAG-expressing A549 cells after incubation with vehicle or 1 μm DEX for 48 h. E, cell morphology and CaD localization in GFP- and GRΔC-expressing A549 cells stained with anti-GFP or anti-FLAG (green in merged image), anti-CaD antibodies (red in merged image), and phalloidin (blue in merged image). Bar, 50 μm.