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Enzymatic activities of His-tag-purified enzymes
| Enzyme | Specific activity, μmol/hr per mg
|
Ratio | ||
|---|---|---|---|---|
| CPSase | ATCase | DHOase | ||
| CAD-wt | 1.4 ± 0.01 | 86.4 ± 4.8 | 47.4 ± 1.8 | 1:60:32 |
| CAD-D90A | 1.3 ± 0.03 | 12.5 ± 0.1 | 31.7 ± 1.4 | 1:10:25 |
| CAD-R269A | 1.2 ± 0.02 | 2.6 ± 0.4 | 29.4 ± 0.9 | 1:2:25 |
