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Kinetic parameters of ATCase in CAD
| Enzyme | Vmax, μmol/hr per mg | [S]0.5Asp, mM |
|---|---|---|
| CAD-wt | 139 ± 6.7 | 0.65 |
| CAD-D90A | 12.5 ± 2.6 | 3.6 |
| CAD-R269A | 3 ± 0.3 | 7.3 |
Because of substrate inhibition, the maximal observed specific activity was defined as the Vmax.
