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. 2008 Dec 5;283(49):33919–33926. doi: 10.1074/jbc.M804240200

FIGURE 2.

FIGURE 2.

Purification of HC1·TSG-6 complex. Pool 1, containing the HC1·TSG-6 complex, from the strong cation exchange-HPLC analysis was subjected to RP-HPLC analysis directly (A) or after treatment with NaOH (B). The asterisk-labeled peaks contained MH+ of m/z 1424.59 and m/z 2241.15, respectively. C, MALDI-TOF MS analysis of peak α from RP-HPLC of the untreated pool 1. The analysis shows that the peak contains the HC1·TSG-6 complex (theoretical monoisotopic MH+: m/z 2174.04). D, MALDI-TOF MS analysis of peak β from RP-HPLC of the NaOH-treated pool 1. The analysis shows that the peak contains the tryptic TSG-6 peptide (22DGIFHNSIWLER33, theoretical monoisotopic MH+: m/z 1486.73), that participates in the cross-link. The analyses demonstrate that HC1·TSG-6 (632VTGVDTD638 cross-linked to 22DGIFHNSIWLER33) has been purified and is present in peak α.