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. 2008 Dec 5;283(49):33919–33926. doi: 10.1074/jbc.M804240200

FIGURE 3.

FIGURE 3.

Purification of HC2·TSG-6 complex. Pool 2, containing the HC2·TSG-6 complex, from the strong cation exchange-HPLC analysis was subjected to RP-HPLC analysis directly (A) or after treatment with NaOH (B). The asterisk-labeled peak contains a non-peptide derived contamination. C, MALDI-TOF MS analysis of peak γ from RP-HPLC analysis of the untreated pool 2 (panel A) shows that the peak contains the HC2·TSG-6 complex (theoretical monoisotopic MH+: m/z 1943.91). D, MALDI-TOF MS analysis of peak λ from RP-HPLC of the NaOH treated pool 2 (panel B) shows that the peak contains the tryptic TSG-6 peptide (22DGIFHNSIWLER33, theoretical monoisotopic MH+: m/z 1486.73), which participates in the cross-link. The analyses demonstrate that HC2·TSG-6 (645VEND648 cross-linked to 22DGIFHNSIWLER33) has been purified and is present in peak γ.