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. 2008 Dec 5;283(49):33919–33926. doi: 10.1074/jbc.M804240200

FIGURE 4.

FIGURE 4.

Tandem MS analyses of the purified HC·TSG-6 complexes. The purified HC1·TSG-6 cross-link (Fig. 2A, peak α)(A and B) and the purified HC2·TSG-6 cross-link (Fig. 3A, peak γ)(C and D) were modified using the CAF derivatization protocol and subjected to tandem MS analyses. The MS/MS fragmentation of the ester bond favors a loss of water from Ser and the derived sequences shown above the spectra are based on dehydrated Ser. Analysis of the fragment ions (supplemental data Tables S1 and S2) correlates with an ester bond between the C-terminal Asp residue of the HCs and Ser28 in TSG-6.