FIGURE 1.

A, time course of phosphorylation levels for different kinases after NMDA stimulation of hippocampal slices. B, summary of the results. ^*, significant changes (p < 0.001, Student's t test); ^**, p < 0.05, Student's t test. C, effect of signaling pathway inhibitors on induced gene expression of Arc, Egr2, Egr4, and Npas4 in qRT-PCRs assays from stimulated and non-stimulated hippocampal slices. Resulting fold changes from the co-application of NMDA and the corresponding inhibitor were normalized by the fold changes obtained with the stimulatory drug only, shown as % (see “Experimental Procedures”). ^*, significant changes (p < 0.05, Student's t test) to 100% of induction.