FIGURE 6.

Requirement of TEF3, not other TEF1 family members, for VEGF-A¹⁶⁵-induced DSCR1-1L expression. A, expression of TEF1, TEF3, TEF4, and TEF5 mRNAs in HUVEC was detected by RT-PCR with the specific primers for TEF1, TEF3, TEF4, and TEF5, respectively. B, HUVEC were transduced with siTEF1A/B/C (siTEF1A, siTEF1B, and siTEF1C), siTEF3A/B (siTEF3A and siTEF3B), siTEF4A/B/C (siTEF4A, siTEF4B, and siTEF4C), siTEF5A/B/C (siTEF5A, siTEF5B, and siTEF5C) or siNeg as a control. Cellular extracts were analyzed by immunoblotting with antibodies against TEF1, TEF3, TEF4, TEG5, DSCR1-1L, and β-actin as the protein equal-loading control. C and D, HUVEC were transduced with siTEF3A siTEF3B individually or in combination. Cellular extracts were analyzed by immunoblotting with antibodies against TEF3 (C) and DSCR1-1L (D). Bottom panels are protein equal-loading controls. The data represent three independent experiments.