FIGURE 8.

TEF3 associated with M-CAT cis element in vitro and in vivo. A, EMSA was performed as above with antibodies against TEF1, TEF3, TEF4, and TEF5, respectively. TEF3 antibody, not other antibodies, supershifted the M-CAT nuclear complex (lane 3). B, CHIP assay. HUVEC extracts were immunoprecipitated with antibodies against TEF1, TEF3, TEF4, TEF5, and IgG as a control. The immunoprecipitates were subjected to PCR to detect the associated DSCR1-1L promoter element (top panel). The bottom is the input DNA without immunoprecipitation. The data represent three independent experiments.