FIGURE 4.

In vivo interaction of Oct25 to Gsc and Mix2 promoters. A, ChIP assays demonstrate that Gsc promoter regions containing either the Oct25 binding site (Gsc-A) or containing the DE and PE (Gsc-B) as well as the Mix2 promoter region spanning the ARE were amplified from chromatin precipitated by an Oct25 antibody. This amplification failed in the absence of antibody (no Ab). B, when Oct25 translation was blocked by Oct25MO, PCR products for Gsc and Mix2 promoter regions were reduced as compared with ctrlMO injections.