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Gsc promoter assays. A, diagram depicting the generation of Gsc promoter-luciferase reporter constructs. B, Oct25 represses Gsc -1500/+3 promoter activities stimulated by overexpression of Xnr1, Smad2, or by incubation with activin A protein. C and D, the same effect is observed for truncated promoters -479/+3(C) and -226/+3(D). E, VP16-XWBSCR11 alone or together with FAST1 stimulates an artificial reporter for DE, which is then repressed by Oct25.
