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Mix2 promoter assays. A and B, Oct25 inhibits Mix2 -712/+13 promoter activity stimulated not only by overexpression of the ligand of nodal signaling, Xnr1 or Xnr5 (A), but also by overexpression of the transducers, Smad2 and/or FAST1 (B). C and D, Oct25 shows a repressive effect on the truncated Mix2 promoters -457/+13 (C) and -221/+13 (D), which are stimulated by Smad2 and/or FAST1.
