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Inhibition of HDAC activity did not alter the inhibitory effect of Oct25 on Gsc and Mix2. A, Gsc expression in wild-type gastrulae. B, circular expression of Gsc in TSA-treated embryos. C, dorsal injection of Oct25 mRNA leads to the disappearance of Gsc expression at the dorsal side in TSA-treated embryos. D and E, Mix2 showed similar expression in control (D) or TSA (E)-treated embryos. F, Oct25 overexpression leads to an inhibition of Mix2 expression in TSA-treated embryos.
