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. 2008 Dec 5;283(49):34273–34282. doi: 10.1074/jbc.M802527200

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Copyright © 2008, The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 7. — HBZ splicing isoform has less ability to destabilize c-Jun than wild-type. A, HBZ-SI promotes degradation of c-Jun less effectively than wild-type. HEK-293T cells were transfected with 0.5 μg of pcDNA3-HA-c-Jun and 3 μg of either pcDNA3-FLAG-HBZ or pcDNA3-FLAG-HBZ-SI. Cell lysates were immunoblotted with anti-HA, anti-FLAG, or anti-α-tubulin antibodies. B, HBZ-SI associates with the proteasome less effectively than wild type. HEK-293T cells were transfected with 8 μg of pcDNA3-FLAG-HBZ or pcDNA3-FLAG-HBZ-SI and then treated with MG132. Cell lysates were immunoprecipitated with an anti-FLAG antibody and then immunoblotted with an anti-α3 antibody. The abundance of α3 and HBZ proteins in whole cell lysates are shown (middle panel and lower panel).