FIGURE 7.
Repression of GRP78/BIP by E2F1 accounts for E2F1-associated chemosensitization of cancer cells. Apoptosis induction after AdERE2F1 infection, 30 μm cisplatin, or combination treatment was analyzed in Hep3B cells by flow cytometry. The percentage of apoptotic cells (as determined by cells with sub-G1 DNA content) 24 h after treatment is as indicated. Semiquantitative reverse transcription-PCR analysis revealed differences in GRP78/BIP transcript levels for each treatment. p73 was used as a positive control. Data were normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) values.