FIGURE 2.

The subcellular localization of HRB is compatible with a role in endocytosis. HeLa cells were fixed and stained for endogenous HRB (in green) and the indicated markers of the clathrin-dependent endocytic pathway (in red). Arrowheads point to structures labeled by both HRB and the indicated marker (bar, 10 μm). Note the high degree of colocalization of HRB with AP-2 and EPS15. HRB also partially colocalizes with clathrin light chain and transferrin receptor, indicating that HRB likely localized in clathrin-coated structures. HRB was also present in a diffuse manner in the nucleus.