FIGURE 4.

HRB colocalizes with endocytosed TI-VAMP. HeLa cells were transfected with pHLuorin-TI-VAMP and then incubated with a GFP antibody at 37 °C to follow the endocytosis of pHLuorin-TI-VAMP. Cells were washed, fixed at the indicated time points, and stained for endogenous HRB (acquired in Cy5 channel, green) and endocytosed anti-GFP antibody (red). Arrowheads point to structure where HRB and pHLuorin-TI-VAMP are both present. Note the good degree of colocalization after 5 min of endocytosis suggesting that the interaction between both proteins may be restricted to the early stages of endocytosis (bar, 10 μm). mGFP, mouse monoclonal anti-GFP antibody.