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. 2008 Dec 5;283(49):34374–34383. doi: 10.1074/jbc.M805916200

FIGURE 8.

FIGURE 8.

Met activation causes nuclear exit of TC45. A, HeLa cells were seeded on coverslips and transfected with Myc-TC45 D/A. 16 h post-transfection, cells were stimulated with 1.5 nm HGF at 37 °C for the indicated times. Coverslips were fixed in 2% PFA and stained with anti-Met AF276 or anti-Myc 9E10 antibodies. Confocal images were taken with a 100× objective and 1× zoom. Yellow staining represents colocalization between endogenous Met (red) and Myc-TC45 D/A (green). B, T47D cells were plated on coverslips and transiently transfected with Myc-TC45 and either WT Met or Met K1110A (kinase dead). 16 h post-transfection, coverslips were fixed in 2% PFA and stained with Met AF276 or Myc 9E10 antibodies. Confocal images were taken with a 100× objective and 1× zoom. Yellow staining represents colocalization between Met (red) and Myc-TC45 D/A (green). The scale bar is indicated in the figure.